HLA polymorphism associated with Susceptibility to Tuberculosis in Mali

Dr. Amadou Koné’s presentation at the 12th conference of the African Society of Human Genetics (AfSHG) and the 1st Congress of the Malian Society of Human Genetics (MSHG).

Introduction: Tuberculosis (TB) is a public health problem caused by bacteria from the Mycobacterium (M.) complex of which M. africanum is responsible for cases in Africa. HLA is important in the immune response especially against tuberculosis infection. Here we study the association of HLA polymorphism and susceptibility to TB infection.

Methods: Subjects were recuited from health centers in Mali : 40 adults with pulmonary active TB and 20 healthy volunteers Mycobacterium tuberculosis complex isolates were spoligotyped to determine TB groups ( M. tuberculosis sensu stricto (N=20) and M. africanum (N = 20)]. HLA (I and Il) was genotyped from PBMC-extracted DNA in participants by SSP-PCR on polymorphic regions of HLA genes and their frequencies were compared between TB groups and healthy subjects.

Results: HLA-B alleles (B*07:02, B*08:01,B*14:02. B*15:03, B*15:10, B*18:01, B*42:01, B*42.02, B*51:01 and B*81:01) were significantly prevalent in the group infected with M. africanum (40-45%) and M. tuberculosis (75%) compared to healthy controls. Some HLA- A (A*02:05, A*34:02, A*66:01 and A*68:02) and DRB1*03:01 alleles were associated with both tuberculosis groups (65-70%). Also, several HLA-DR variants were associated with M. tuberculosis sensu stricto and not with M. africanum.

 Discussion and conclusion: We found that HLA-A and HLA-B specific alleles are more common in individuals with tuberculosis compared to healthy controls. However it is necessary to extend this study to validate these results that could be used in the development of anti-TB vaccines.

 Conflict of interest disclosure: the authors declare no conflict of interest in terms of scientific, financial and personal.

Keywords: Tuberculosis, Mycabacterium africanum, HLA, Mali.

A. Kone, M. T. Diakite, H. B. Diarra, A. Coulibaly, D. Diallo, B. Diarra’, K. Cohen, B. Baya, S. Diabate, B. Kone, M. Sanogo, A.C.G. Togo, Y. S. Sarro, N. Coulibaly, O. Kodio, C.A. Achenbach, R.L. Murphy, JL. Holl, S. Siddiqui, w. Bishai, M. Maiga, S. Diallo, M. Diakite and S. Doumbia.

University of Sciences, Techniques and Technologies of Bamako (USTTB)

Northwestern University, Chicago, Illinois, USA

National Institute of Allergic and Infectious Diseases, Rockville, Maryland, USA

Name of institution: Unit of Molecular Biology, SEREFO-University Clinical Research Center (SEREFO-UCRC), University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali.

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Ibrahima DIA

Communication Officer of the University Clinical Research Center (UCRC).

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